Salinity-Induced Early Activation of Flavonoid Biosynthesis Genes in Coriander Coriandrum sativum L.: A qRT-PCR-Based Analysis of CHS-Mediated Stress Response
Department of Clinical Laboratory Sciences, College of Pharmacy, Tikrit University, Tikrit, Iraq.
Research Article
International Journal of Life Science Research Archive, 2026, 10(02), 150-158.
Article DOI: 10.53771/ijlsra.2026.10.2.0047
Publication history:
Received on 06 May 2026; revised on 12 June 2026; accepted on 15 June 2026
Abstract:
CHS is a pivotal regulatory enzyme in the plant flavonoid biosynthetic pathway, and it has been closely linked to plant salinity stress defense response. The present study was designed to assess the CHS gene early transcriptional response to short-term salinity stress in coriander Coriandrum sativum L. leaves. The plants of Coriander were treated with 150 mM NaCl for 24 hours and 3 biological replicates were set for each experimental group. Gene expression was quantified by using RT-qPCR, normalizing to the housekeeping gene, GAPDH. The relative amount of transcripts was determined by the 2−ΔΔCt method. The results showed upregulation of CHS transcript levels after salinity stress, and a calculated fold change of 4.20 after salinity stress compared with control plants which were not treated. Under salt stress, the mean cycle threshold (Ct) value of the CHS gene decreased, from 18.72 in the control group to 16.58, while the reference gene GAPDH value was almost similar with the values of 15.45 and 15.38, respectively. This is an early transcription activation of the flavonoid biosynthetic pathway in response to salinity stress. The results indicate that the CHS is a key regulator in the activation of antioxidant defense mechanisms in C. sativum against salt stress and could be considered as a potential molecular marker for further research on enhancing resistance to salt stress.
Keywords:
Coriandrum sativum L; CHS gene; RT-qPCR; Flavonoid Biosynthesis
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