Bioactive compounds and in vitro evaluation of antioxidant capacities, antimicrobial properties, and effectiveness of cytotoxicity of three edible mushrooms from Egypt

Ghada A. Youssef 1, *, Marwa M. Naguib 1 and Doha M. Beltagy 2

1 Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria, 21431, Egypt.
2 Department of Chemistry, Division of Biochemistry, Faculty of Science, Damanhour University, Damanhour, Egypt.
 
Research Article
International Journal of Life Science Research Archive, 2023, 04(02), 018–030 .
Article DOI: 10.53771/ijlsra.2023.4.2.0051
Publication history: 
Received on 23 February 2023; revised on 05 April 2023; accepted on 08 April 2023
 
Abstract: 
Many edible mushrooms have been evaluated as new therapeutic alternatives with various activities. We focused this work to estimate the antioxidant, antimicrobial and cytotoxicity effects of three edible aqueous mushroom extracts: Agaricus bisporus (white button mushroom), Pleurotus ostreatus (pearl oyster mushroom), and Pleurotus pulmonarius (Phoenix mushroom). The biochemical analysis of extracts revealed marked variations for total carbohydrate, protein, phenolic, and flavonoid contents. The three extracts were tested for antioxidant activities using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging assay. Pleurotus ostreatus had a potential antioxidant capacity with lowest IC50 value (13.26 µg/mL). Distinct variable degrees of antimicrobial activity were detected against five reference microbial strains, exerting the minimum inhibitory concentration (MIC) ranging from 28 to 89 mg/ml. Agaricus bisporus exhibited the highest cytotoxicity against EAC cells with cell viability 68% and showed an evidenced inhibitory action against human breast cancer cell lines (MCF-7) based on Sulforhodamine B (SRB) assay.
 
Keywords: 
Mushroom extract; Bioactive; Antioxidant; Antimicrobial; Cytotoxic
 
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Copyright information: 
Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0