Biosynthesis of gallic acid and tannase from Dacryodes edulis (African Pear) seeds using Aspergillus niger SCSGAF0145 by submerged fermentation
1 Department of Biological Science, School of Pure and Applied Sciences, Lagos State Polytechnic, Ikorodu, Lagos State, Nigeria.
2 Department of Chemical Science, School of Pure and Applied Sciences, Lagos State Polytechnic, Ikorodu, Lagos State, Nigeria.
Research Article
International Journal of Life Science Research Archive, 2022, 03(01), 085–092.
Article DOI: 10.53771/ijlsra.2022.3.1.0076
Publication history:
Received on 10 July 2022; revised on 29 August 2022; accepted on 31 August 2022
Abstract:
Aspergillus niger SCSGAF0145 previously isolated and identified by our group as a tannase producer from soil was grown under submerged fermentation using pear seed as source of tannic acid. Crude tannase was precipitated out of the cell free broth and purified by gel filtration gave a purified enzyme with specific activity of 6.65 U/mg, with a final yield and purification fold of 42.08% and 5.94 respectively. The deduced Vmax of the purified tannase was 17.57 U/mL and Km was 1.59 mg/mL. The optimal temperature and pH of the enzyme using tannic acid as sole carbon source was 40 °C and 5.5 respectively. Conclusively, pear seed is a good substrate for gallic acid and tannase production by Aspergillus niger SCSGAF0145 and tannase secreted can be explored in various industrial processes.
Keywords:
Aspergillus niger SCSGAF0145; Tannase; Gallic acid; Pear seed
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