Anti-oxidant and cytotoxic effect of collagen extracted from local buffalo
1 Department of Molecular and Medical Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq.
2 Department of Animal Production, College of Agriculture, University of Misan, Maysan, Iraq.
3 Department of Plant Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq.
Research Article
International Journal of Science and Technology Research Archive, 2024, 06(01), 037–041.
Article DOI: 10.53771/ijstra.2024.6.1.0109
Publication history:
Received on 26 November 2023; revised on 07 February 2024; accepted on 10 February 2024
Abstract:
This study about aimed to consider the DPPH radical scavenging recreation and cytotoxicity potentials of collagen extracted from buffalo. The result of antioxidant (DPPH) indicated that collagen possessed the ability to scavenge free in a concentrations dependant manner. They were (67.86±3.65, 54.44± 2.05, 47.92 ±0.53, 44.29±1.80 and 18.02±5.74%) for (200,100,50,25 and 12.5 mg\ml) respectively. the results of cytotoxicity assay match the antioxidant results, the cell viability decrease by concentrations increasing to be 40.20± 3.06, 48.64± 1.48, 61.57± 1.33, 71.14± 0.82 and 83.21± 1.10% for400,200,100,50 and 25 μg/ml respectively within IC50 of 98.54 μg/ml. A little effect on viability of normal cells were observed after collagen treatment (72.33±2.41, 76.27±5.12, 83.17±1.31, 94.56±1.51 and 94.13±0.99 %) for 400,200,100,50 and 25 μg/ml respectively within IC50 of 122 μg/ml.
Keywords:
Collagen; DPPH; Antioxidant; Anticancer
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