Assessment of Hepatitis C virus genotypes in regular dialysis patients of Khyber Pakhtunkhwa, Pakistan
1 State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China 100193.
2 School of Life Sciences Anhui Normal University.
3 Department of Zoology, Kohat University of Science and Technology Kohat, 26000 Pakistan.
Research Article
International Journal of Science and Technology Research Archive, 2021, 01(01), 001-007.
Article DOI: 10.53771/ijstra.2021.1.1.0011
Publication history:
Received on 18 May 2021; revised on 22 June 2021; accepted on 24 June 2021
Abstract:
Globally viral hepatitis is a major health problem. HCV is a causative agent of hepatitis and is responsible for acute and chronic hepatitis leads to cirrhosis and hepatocellular carcinoma. This study was carried out to know the HCV genotypes in Dialysis patients in NWFP (Pakistan). The age ranged from 15-65 years. During this study a total of 63 samples were collected and were analyzed for HCV genotypes. RNA was extracted from whole blood; reverse transcribed into cDNA and was subjected to multiplex PCR. Of these 63 samples, 14 were genotyped as genotype 3a was found in 9(64.28%) patients, followed by genotype 3b (21.42%) in 3 and 2a in 2(14.28%) patients. Three positive samples remained untyped. In age group 31 to 40 years, the number of positive patients were comparatively greater.
Keywords:
Hepatitis C virus; Genotypes; Dialysis; PCR
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