Influence of Candida albicans biofilms on the resistance of Salmonella typhi to antibiotics
1 Department of Biochemistry, Faculty of Sciences, University of Douala, P.O box: 24157 Douala, Cameroon.
2 The Health and Pathologies Priority Research Center, Institut of Medical Research and of Plant Medicinal Studies, P.O box: 13033, Yaounde, Cameroon.
3 Department of Microbiology, Faculty of Sciences, University of Yaounde I, P.O box: 337 Yaounde, Cameroon.
4 Department of Biochemistry, Faculty of Sciences, University of Yaounde I, P.O box: 812 Yaounde, Cameroon.
5 Department of Obstetrics and Gynecology, Third Affiliated Hospital of Chongqing Medical University, Chongqing, 401120, China.
Research Article
International Journal of Science and Technology Research Archive, 2023, 04(01), 135–142.
Article DOI: 10.53771/ijstra.2023.4.1.0176
Publication history:
Received on 20 November 2022; revised on 28 December 2022; accepted on 31 December 2022
Abstract:
In many countries around the world, Candida albicans - Salmonella typhi co-infection would represent a real health problem according to the potential interactions that exist between both pathogens. The aim of this work was to study the impact of C. albicans on the resistance of S. typhi to antibiotics and to determine the influence of fungal biofilms on its growth. Reactivated clinical isolates of S. typhi (n = 5) and C. albicans (n = 5) were co-cultured on specific media containing or free of MgCl2. The colonies obtained were analysed by microscopy and an antibiogram was performed from it. The analysis indicated that in case of simultaneous growth of S. typhi and C. albicans, there is improvement in fungal biofilm formation. But when S. typhi infection is posterior to that of C. albicans, fungal biofilms stimulated in vitro are disorganized. Moreover, the sensibility of S. typhi to antibiotherapy in-vitro appeared to decrease in the presence of C. albicans , by approximately 100% with ofloxacin, amoxicillin, doxycycline, ciprofloxacin and ceftriaxone, 87.55% with cefoxitime and 80% with colistin. Altogether, the results of the present study suggest that the co-culture of both pathogens improves the infectious capacity and resistance of S. typhi to antibiotherapy through fungal biofilm.
Keywords:
S. typhi; C. albicans; Biofilms; Antibiotics; Resistance
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