Antibacterial and anti-biofilm activities of grape seed oil and black seed oil against Uropathogenic Escherichia coli isolated from women with urinary tract infections

Shahad Imad Hameed *

Department of Biology, college of science, University of Baghdad, Baghdad, Iraq.
* Corresponding Author; Email: Shahad.emad@sc.uobaghdad.edu.iq
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Research Article
International Journal of Science and Technology Research Archive, 2026, 11(02), 001–009.
Article DOI: 10.53771/ijstra.2026.11.2.0037
Publication history: 
Received on 25 August 2026; revised on 30 September 2026; accepted on 02 October 2026
 
Abstract: 
This study was conducted to assessed the antibacterial and anti-biofilm activity of grape seed and black seed oils against clinical isolates of E. coli which obtained from women suffer from urinary tract infections. Different concentrations of both oils were tested against twelve isolates of E. coli. Biofilm inhibition was measured at doses between 6.25 and 200 µg/mL to determine the anti-biofilm activity. The anti-biofilm capacity of both oils was concentration-dependent, and the inhibitory effect enhanced with oil concentration. Black seed oil demonstrated 89.37% biofilm inhibition at 200 µg/mL, whereas grapeseed oil demonstrated a maximum inhibition of 95.44%. Significant variations between the measured concentrations for each oil were shown by statistical analysis. In conclusion, both oils demonstrated a supportive in vitro anti-biofilm action against clinical isolates of E. coli, with grapeseed oil exhibiting a greater percentage of inhibition than black seed oil.
 
Keywords: 
Antibacterial, Anti-biofilm, E. coli, Grape seed oil (GSO), Black seed oil (BSO).
 
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Copyright information: 
Copyright © 2026 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0